5-AMINO-1MQ (50mg)

Product Overview

5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule quinolinium derivative identified as a selective, cell-permeable inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic methyltransferase enzyme that catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine (SAM) as the methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosyl-L-homocysteine (SAH).

Specifications

• Quantity: 50mg lyophilized powder
• Purity: ≥98% (HPLC verified)
• Manufacturing: Produced in a GMP-compliant facility in the USA
• Molecular Weight: 159.21 g/mol
• Molecular Formula: C₁₀H₁₁N₂⁺
• CAS Number: 42464-96-0
• PubChem CID: 950107
• Appearance: White to off-white lyophilized powder
• Solubility: Soluble in water and DMSO
• Shelf Life: 36 months when stored properly

Storage & Handling

• Store at -20°C for long-term storage
• Stable at 2-8°C for short-term use
• Protect from light and moisture
• Keep in original sealed container until use

Mechanism of Action

5-Amino-1MQ competitively inhibits NNMT by occupying the nicotinamide substrate-binding pocket. NNMT methylates nicotinamide (a precursor to NAD+) into 1-methylnicotinamide, effectively diverting nicotinamide away from the NAD+ salvage pathway. By inhibiting NNMT, 5-Amino-1MQ increases intracellular nicotinamide availability for conversion to NAD+ via nicotinamide phosphoribosyltransferase (NAMPT). This results in elevated NAD+ levels, which serve as a critical cofactor for sirtuin deacetylases (SIRT1-7) and poly(ADP-ribose) polymerases (PARPs). NNMT inhibition also reduces SAM consumption, shifting the SAM/SAH ratio and altering cellular methylation dynamics. In adipocyte models, NNMT knockdown or pharmacological inhibition increases energy expenditure gene expression, including uncoupling proteins and PGC-1α targets.

For Laboratory Use Only. Laboratory Use Only.

Quality Assurance: Each lot undergoes independent third-party LCMS/MS analysis. Certificates of Analysis are available on the product page.